Differential pulse adsorptive stripping voltammetric determination of methotrexate using a functionalized carbon nanotubes-modified glassy carbon electrode |
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Authors: | Geiser G Oliveira Bruno C Janegitz Valtencir Zucolotto Orlando Fatibello-Filho |
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Institution: | 1. Department of Chemistry, Center for Science and Technology, Federal University of S?o Carlos, P. Box 676, 13560-970, S?o Carlos - SP, Brazil 2. Nanomedicine and Nanotoxicology Group, S?o Carlos Institute of Physics, University of S?o Paulo, 13566-590, S?o Carlos - SP, Brazil
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Abstract: | A glassy carbon electrode (GC) containing multiwalled functionalized carbon nanotubes (MWCNTs) immobilized within a dihexadecylhydrogenphosphate film (DHP) is proposed as a nanostructured platform for determination of methotrexate (MTX) concentration (a drug used in cancer treatment) using differential pulse adsorptive stripping voltammetry (DPAdSV). The voltammograms for a MTX solution using MWCNTs-DHP/GC electrode presented an oxidation peak potential at 0.98 V vs. Ag/AgCl (3.0 mol L?1 KCl) in a 0.1 mol L?1 sulphuric acid. The apparent heterogeneous electron transfer rate constant of 0.46 s?1 was calculated. The recovery area of 2.62×10?9 mol cm2 was also obtained. Under the optimal experimental conditions, the analytical curve was linear in the MTX concentration range from 5.0×10?8 to 5.0×10?6 mol L?1, with a detection limit of 3.3×10?8 mol L?1. The MWCNTs-DHP/GC electrode can be easily prepared and was applied for the determination of MTX in pharmaceutical formulations, with results similar to those obtained using a high-performance liquid chromatography comparative method. |
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