氟环唑外消旋体在纤维素-三(3,5-二甲基苯基氨基甲酸酯)手性固定相上的高效液相色谱法拆分

在纤维素-三(3,5-二甲基苯基氨基甲酸酯)（CDMPC）手性固定相上,分别采用正相、反相及极性有机相色谱模式对氟环唑外消旋体进行了拆分,并考察了流动相组成在手性识别中对手性分离的影响。氟环唑在Chiralcel OD-H手性色谱柱（填充CDMPC手性固定相）上采用反相色谱模式，以甲醇-水(体积比为80∶20)为流动相，获得了最佳的拆分,其两对对映异构体的分离度Rs分别为1.64和6.50。

Chiral separation of racemic epoxiconazole Oil cellulose tris(3,5-dimethylphenylcarbamate)chiral stationary phase using high performance liquid chromatography

The two pairs of enantiomers of epoxiconazole were resolved using normal phase, reversed-phase and polar organic solvent high performance liquid chromatography on cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) chiral stationary phase, separately. The effects of different mobile phase compositions on the retention factor, separation factor and resolution in the chiral separation of epoxiconazole were investigated. It can be baseline separated on a Chiralcel OD-H column packed with CDMPC chiral stationary phase when methanol-water (80∶20, v/v) was used as the mobile phase, the resolutions of the two pairs of enantiomers were 1.64 and 6.50, respectively.