| 高效液相色谱法测定地榆中金丝桃甙的含量 |
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| 引用本文： | 沙明,曹爱民,王冰,刘春丽,耿杰,刘威.高效液相色谱法测定地榆中金丝桃甙的含量[J].色谱,1998,16(3):226-228. |
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| 作者姓名： | 沙明 曹爱民 王冰 刘春丽 耿杰 刘威 |
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| 作者单位： | 辽宁中医学院，丹东市第二医院，普兰店市中医院 |
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| 摘 要： | 采用高效液相色谱法测定了地榆中金丝桃甙的含量。色谱柱为Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ柱，以甲醇－０．０２５ｍｏｌ／Ｌ磷酸（５０５０）为流动相，检测波长３７０ｎｍ，测定组分与其它组分的色谱峰达到基线分离。加样回收率平均值为９８．７％，ＲＳＤ为１．９％。为控制中药地榆的内在质量提供了新的测定指标和可靠的方法。
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| 关 键 词： | 高效液相色谱法 金丝桃甙 地榆 |
Determination of hyperin in Sanguisorba Officinalis L. by high performance liquid chromatography] |
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| M Sha,A Cao,B Wang,C Liu,J Geng,W Liu.Determination of hyperin in Sanguisorba Officinalis L. by high performance liquid chromatography][J].Chinese Journal of Chromatography,1998,16(3):226-228. |
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| Authors: | M Sha A Cao B Wang C Liu J Geng W Liu |
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| Institution: | Liaoning College of Traditional Chinese Medicine, Shenyang, 110032. |
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| Abstract: | An HPLC method for the determination of hyperin in Sanguisorba officinalis L. was investigated. 0.5 g of dried powdered crude drug was weighed accurately in a 50 mL volumetric flask added with 40 mL methanol, supersonicated for 30 min and then made up to volume with methanol. The sample solution was separated on a Shim-pack ODS column(6.0 mm i.d. x 150 mm, 5 microns) with methanol-0.025 mol/L phosphoric acid (adjusted to pH 3.0 by using triethylamine)(50:50) as the mobile phase and detected at UV-370 nm. Calibration curve of hyperin was obtained for the concentration range of 0.046-0.2 microgram. The corresponding regression equation was Y = 154 x 10(4) X-0.28 x 10(4), r = 0.9998. The recovery was 98.7% and relative standard deviation was 1.9%. The results showed that this method is simple, specific and accurate. |
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| Keywords: | NULL |
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