反相离子对高效液相色谱法测定动物血浆中的恩诺沙星及其代谢物

建立了用反相离子对ＨＰＬＣ测定动物血浆中恩诺沙星（ＥＲＦＸ）及其代谢产物环丙沙星（ＣＰＦＸ）浓度的方法。血浆中药物用二氯甲烷萃取，选用ＯＤＳ柱，甲醇－四丁基氢氧化铵溶液为流动相，吡哌酸（ＰＰＡ）作内标，检测波长２７２ｎｍ。方法简便、快速、灵敏、准确，适用于ＥＲＦＸ及其代谢物ＣＰＦＸ血药浓度的测定和药代动力学研究。并首次测定了黄牛血浆中的ＥＲＦＸ和ＣＰＦＸ。

Determination of enrofloxacin and it's main metabolite in animal plasma by reversed-phase ion-pair high performance liquid chromatography]

This paper describes a method for determining enrofloxacin (ERFX) and it's main metabolite ciprofloxacin(CPFX), in animal plasma by RP-IPLC simultaneously. Dichloromethane was utilized as an extracting agent of plasma. Chromatographic conditions were ODS column with methanol-25 mmol/L tetrabutylammonium hydroxide (20:80, V/V, pH 3.0) as mobile phase, pipemidic acid as the internal standard and UV detection at 272 nm. With the method established in this paper, the work is done simply, rapidly, sensitively and accurately. It was actually adapted to determine ERFX and it's metabolite CPFX concentrations in plasma and pharmacokinetic studies, and to determine ERFX and CPFX contents in calves plasma successfully for the first time.