高效液相色谱法同时测定快安抗感冒液中5种组分的含量

 采用高效液相色谱法（HPLC）同时测定快安抗感冒液中对乙酰氨基酚、咖啡因、盐酸甲基麻黄碱、马来酸氯苯那敏和愈创木酚甘油醚的含量。色谱柱为NOVA－PAKC_18柱，流动相为V（已腈）：V（0．03mol／L磷酸氢二铵）：V（0．004mol／L庚烷磺酸钠）：V（三乙胺）=12：44：43．5：0．5，并用磷酸调节pH值至3．0；检测波长220nm，柱温35℃。在此色谱条件下，各组分均获得良好分离。平均回收率为98．0％～100．3％，相对标准偏差小于1．8％。

Simultaneous determination of 5-component contents in kuaian anticold solution by high performance liquid chromatography]

A reversed-phase, isocratic high performance liquid chromatographic method has been successfully used to separate and determine the 5 components simultaneously in Kuaian anticold solution. In the anticold solution, paracetamol, caffeine, DL-methylephedrine HCl, chlorpheniramine maleate and guaiacol glyceryl ether were analyzed on a NOVA-PAK C18 column (150 mm x 3.9 mm i.d., 5 microns) at 35 degrees C with V(acetonitrile):V(0.03 mol/L ammonium dihydrogen phosphate):V(0.004 mol/L sodium heptyl sulfonate):V(triethylamine) = 12:44:43.5:0.5 with pH adjusted to 3.0 by orthophosphoric acid as the mobile phase. The detection was performed at 220 nm and the injection volume was 15 microL. For the 5 components mentioned above the calibration curves were linear in a certain range. The recoveries were (99.6 +/- 1.3)%, (98.4 +/- 1.7)%, (98.0 +/- 1.5)%, (98.5 +/- 1.8)%, (100.3 +/- 1.7)% (n = 5) respectively.