毛细管区带电泳法分析头孢菌素类药物的纯度

 摘要：提出了以pH9.20的20mmol/L硼酸缓冲溶液、pH6.86的20mmol/L磷酸缓冲溶液和pH2.05的50mmol/L磷酸缓冲溶液作为背景电解质分析9种头孢菌素类抗生素药物纯度的毛细管区带电泳方法。用内部归一化法定量，方法简单快速。讨论了方法的优点和局限性。

Purity Analysis of Cephalosporins with Capillary Zone Electrophoresis

A capillary zone electrophoresis method is proposed for the purity determination of nine cephalosporin drugs. A background electrolyte comprising of either 20mmol/L pH 9.20 borate buffer or 20mmol/L pH 6.86 phosphate buffer was used for most drugs studied except for cefaloridine which formed neutral molecules at these pH values. For it 50 mmol/L pH 2.05 phosphate buffer was used instead. Internal normalization method was employed for quantitation. The method is simple, rapid and versatile. Analysis was completed within 8min. The merits and limitations of the method were also discussed.