| 高效液相色谱法在双酶协同作用酶解淀粉制取麦芽低聚糖工艺研究中的应用 |
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| 引用本文： | 吴红京,唐根源,李昊,李志达.高效液相色谱法在双酶协同作用酶解淀粉制取麦芽低聚糖工艺研究中的应用[J].色谱,1999,17(2):208-210. |
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| 作者姓名： | 吴红京 唐根源 李昊 李志达 |
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| 作者单位： | 中国科学院福建物质结构研究所!福州，350002，中国科学院福建物质结构研究所!福州，350002，福州大学生物与食品科学工程系!福州，350002，福州大学生物与食品科学工程系!福州，350002 |
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| 基金项目： | 福建省科委重点科研项目基金 |
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| 摘 要： | 介绍了高效液相色谱在双酶协同作用酶解制取麦芽低聚糖工艺研究中的应用。以C18柱为分离柱，水作流动相，利用折光检测器来检测麦芽低聚糖产品中的7种糖，同时评估了补加酶量与麦芽低聚糖中麦芽三糖至六糖含量的关系，并测定了二次确认实验中麦芽低聚糖产品中各糖的含量。
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| 关 键 词： | α-淀粉酶 高效液相色谱法 麦芽低聚糖 木薯淀粉 协同作用 异淀粉酶 |
Application of High Performance Liquid Chromatography to the Study on the Production of Maltooligosaccharide by Synergistic Action of Two-Enzymes |
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| Wu Hongjing and Tang Genyuan.Application of High Performance Liquid Chromatography to the Study on the Production of Maltooligosaccharide by Synergistic Action of Two-Enzymes[J].Chinese Journal of Chromatography,1999,17(2):208-210. |
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| Authors: | Wu Hongjing and Tang Genyuan |
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| Institution: | Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002. |
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| Abstract: | This paper describes the application of high performance liquid chromatography to the study on the production of maltooligosaccharide by synergistic action of two-enzymes. Seven sugars in products were separated on a column of Nucleosil C18 with water as the mobile phase and determined refractometrically. Meanwhile, for the preparation of maltooligosaccharide, the effect of supplementing rate of the two enzymes on the content of maltooligosaccharide in products is examined in this paper. The optimun technolgical conditions were obtained by using the orthogonal design and the amount of the two enzymes added was determined. Thus more than 70% of M3-M6 maltooligosaccharide in product was obtained. |
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| Keywords: | NULL |
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