气相色谱-三重四极杆质谱法同时测定巴氏杀菌乳中9种香精成分

建立了气相色谱-三重四极杆质谱（GC-MS/MS）同时检测巴氏杀菌乳中9种香精成分（二氢香豆素、香兰素、香豆素、乙基香兰素、甲基香兰素、7-甲基香豆素、7-甲氧基香豆素、7-乙氧基-4甲基香豆素和环香豆素）的分析方法。巴氏杀菌乳样品用乙醇溶液萃取，低温涡旋离心，取上清液过0.22 μm滤膜，以DB-5MS色谱柱分离，在MRM模式下测定，基质曲线外标法定量。实验结果表明，9种香精成分在1~200 μg/L范围内呈良好线性，线性相关系数（R²）均大于0.997，方法的检出限为0.002~0.1 μg/kg，定量限为0.001~2 μg/kg，平均回收率为90.3%~110.6%，日内、日间精密度均小于10%。该方法前处理简单快速，检测准确度和灵敏度高，可用于巴氏杀菌乳中9种香精成分的同时检测。

Simultaneous determination of nine flavor compounds in pasteurized milk by gas chromatography- triple quadrupole mass spectrometry

A gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method was established for the simultaneous determination of nine flavor compounds (hydrocoumarin, vanillin, coumarin, ethyl vanillin, methyl vanillin, 7-methylcoumarin, 7-methyoxycoumarin, 7-ethoxy-4-mrthyl coumarin, pyranocoumarin) in pasteurized milk. The samples were extracted with ethanol and subjected to whirlpool oscillation. After centrifugation, the supernatant was filtered through a membrane, and the components were separated on a DB-5MS column. The nine analytes were detected by GC-MS/MS in the multiple reaction monitoring (MRM) mode and quantified by a matrix-matched external standard method. Good linearities in the range of 1-200 μg/L were observed for the nine flavor compounds. The linear correlation coefficients (R²) were greater than 0.997. The limits of detection and limits of quantification were 0.002-0.1 μg/kg and 0.001-2 μg/kg, respectively. The average recoveries were 90.3%-110.6%. Both the intra-day and inter-day RSDs were less than 10%. The developed method is simple, rapid, accurate and sensitive, and can be used for the simultaneous determination of the nine flavor compounds in pasteurized milk.