计算机辅助一清片径向展开薄层色谱定性与定量方法初探

径向薄层色谱法（RTLC）是一种将样品呈圆形或环状点样，然后将展开剂从中心向外沿径向输送的薄层色谱方法。在一定的展开距离内，RTLC是一种分离快速、扩散小、不拖尾的高效分离和半制备方法。目前关于RTLC分离的报道较多，但对RTLC定性和定量的报道较少。该文采用自编程方式结合图像处理软件，实现RTLC图像数字化处理，形成RTLC数字化图谱，并对RTLC进行定性与定量方法学验证，建立了一清片RTLC定性与定量方法。辅助计算机技术，对一清片径向展开薄层色谱定性与定量方法进行初探。结果表明，该方法用于RTLC定性的可靠度较好，但用于RTLC的定量，仍有不足之处，这可能与实验环境条件等有关。通过搭载更好的分离和数据采集设备，RTLC会有适合的用武之地。计算机图像处理和自编程方法的结合给了RTLC和其他一些分析相关的技术广阔的发展前景。相信未来在计算机技术的辅助下，薄层色谱技术会迈上一个新的台阶。

Primary study on computer-aided qualitative and quantitative radial thin-layer chromatography methods for analysis of Yiqing tablets

Radial thin-layer chromatography (RTLC) is a form of chromatography in which the sample and developing agent/solvent are applied at the edge of a circle on the silica-layered TLC plate. The sample components are radially transported from the center of the circle toward the periphery by the solvent flow. RTLC is an efficient separation and semi-preparation method, with the advantages of fast separation, low diffusion, and absence of tailing at a certain distance. Although RTLC isolation has been well documented in the literature, there are only a few qualitative and quantitative reports on this technique. In this study, a self-programming module combined with image processing software was adopted for digital processing of RTLC images, and a digital atlas was created. Qualitative and quantitative verification of RTLC was then conducted. With the aid of computer technology, qualitative and quantitative methods of radially expanded TLC for Yiqing tablets were investigated. The results showed that the qualitative RTLC method has good reliability, but the quantitative RTLC method has inherent drawbacks, which may be associated with the experimental environment. We strongly believe that better separation and data acquisition equipment, as well as the combination of computer image processing and programming methods, will open up infinite possibilities of analysis/separation using RTLC-based techniques. We also believe that TLC will take a new step in the future with the assistance of computer technology.