反相高效液相色谱法测定普通鹿蹄草中的黄酮苷类成分

建立了普通鹿蹄草中5种黄酮苷(金丝桃苷、异槲皮苷、2″-O-没食子酰基金丝桃苷、槲皮素-3-O-呋喃阿拉伯糖苷和槲皮苷)的高效液相色谱检测方法。采用Zorbax Extend-C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-水(体积比14∶86)为流动相,在波长为350 nm处检测。在此条件下,样品中5种黄酮苷分离良好且无杂质峰干扰,低、中、高浓度下的回收率为96.3%~104.2%,相对标准偏差（RSD）小于5%。该方法简便快速、结果准确、重现性好,可以作为普通鹿蹄草药材及相关制剂质量控制的一个有效方法。

Determination of Flavonoid Glycosides in Pyrola decorate by Reversed-Phase High Performance Liquid Chromatography

A reversed-phase high performance liquid chromatographic method was developed for the simultaneous determination of five flavonoid glycoside components, namely hyperin, isoquercitrin, 2"-O-galloylhyperin, quercetin 3-O-alpha-L-arabinofuranoside and quercitrin in Pyrola decorate. After being immersed in 70% (v/v) ethyl alcohol for 6 h, samples were ultrasonically extracted for 30 min. The separation was carried out on a Zorbax Extend-C18 column (250 mm x 4.6 mm, 5 microm) using acetonitrile-water (14 : 86, v/v) as mobile phase at a flow rate of 1.0 mL/min. The absorbance was monitored at 350 nm and the column temperature was 35 degrees C. All analytes showed good linearity within the test ranges and the recoveries were 96.3% - 104.2%. Satisfactory precisions were obtained with relative standard deviations less than 5%. The method was simple, accurate and reproducible in the application to the determination of 20 commercial samples of Pyrola decorata collected from different regions of China. It can be used for the quality control of both plant materials and preparations of Pyrola decorate.