键合型手性固定相的研究进展

手性分离在生物医药等领域具有重要意义。高效液相色谱(HPLC)因其经济、快速、高效等特点被广泛应用于手性化合物的分离分析中。手性固定相(CSP)是HPLC实现手性分离的核心,而制备有效CSP的关键在于手性选择剂的筛选。近年来,大量文献报道了新型CSPs的制备,其中键合型CSPs因具有溶剂耐受性和较高稳定性等优点受到了广泛关注。该文对近年来以手性单分子、多糖、环糊精、大环抗生素、冠醚、杯芳烃及生物碱等为手性选择剂制备的新型键合型CSPs进行了归纳整理,并对其发展前景进行了展望。

Research progress of bonded chiral stationary phases

Chiral separation is important in biological medicine and other fields. High performance liquid chromatography (HPLC) is widely used in chiral separation and analysis for its economic, rapid and efficient characteristics. Chiral stationary phase (CSP) is the key to achieve chiral resolution in HPLC. Meanwhile, the key to preparing effective CSP is the screening of chiral selector. In recent years, a lot of CSPs with different chiral selectors had been prepared. Silica gel immobilized CSP is especially attached great attention because of its high solvent tolerance and stability. In this paper, the new type of CSPs prepared by using chiral single molecules, polysaccharides, cyclodextrins, macrocyclic antibiotics, crown ethers, calixarenes and alkaloids as chiral selectors are summarized, and the development prospect of immobilized CSPs are also discussed.