固相萃取-高效液相色谱法测定啤酒中的黄腐酚

采用Waters Sep-Pak C18固相萃取小柱对啤酒样品进行分离纯化,建立了啤酒中黄腐酚的固相萃取-高效液相色谱检测方法。选用色谱柱Zorbax Eclipse XDB-C18柱(250 mm×4.6 mm,5 μm),以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,柱温25 ℃,流速0.4 mL/min,检测波长370 nm。在此条件下,黄腐酚分离良好且无杂质峰干扰,在0.5～500 μg/L的范围内线性关系良好(r21),在高、中、低浓度下的加标回收率为91.21%～95.58%,相对标准偏差小于2%。方法的检出限为0.24 μg/L,定量限为0.80 μg/L。该方法简便快速、结果准确、重现性好,是检测啤酒中黄腐酚含量的有效方法。

Determination of xanthohumol in beer by solid-phase extraction-high performance liquid chromatography

A method for the determination of xanthohumol in beer using solid-phase extraction-high performance liquid chromatography (SPE-HPLC) has been developed. A Waters Sep-Pak C18 column was used to extract and clean-up the sample. The separation was achieved on a reversed-phase Agilent Zorbax Eclipse XDB-C18 (250 mm×4.6 mm, 5 μm) in a linear gradient, and the mobile phases were consisted of water (containing 0.1% formic acid) (A) and methanol (B) with a flow rate of 0.4 mL/min. In addition, the column temperature was maintained at 25 ℃. The detection wavelength was set at 370 nm. There was a good linear relationship (r21) in the range of 0.5~500 μg/L. The average recoveries were between 91.21% and 95.58% with the relative standard deviations less than 2%. The limit of detection was 0.24 μg/L and the limit of quantification was 0.80 μg/L. It was proved to be a convenient and accurate method for the analysis of xanthohumol content in beer.