| 高效液相法测定螺旋霉素发酵液中的有机酸 |
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| 引用本文： | 李友元,陈长华,陶萍.高效液相法测定螺旋霉素发酵液中的有机酸[J].色谱,2002,20(1):46-48. |
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| 作者姓名： | 李友元 陈长华 陶萍 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
2. 上海交通大学分析测试中心,上海,200030 |
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| 摘 要： | 采用高效液相法在Zorbax 30 0 SBC18柱 (5 μm ,4 6mmi.d .× 15cm)上以 0 0 1mol/L磷酸缓冲液(pH 2 32 )和甲醇的二元流动相梯度分离测定了螺旋霉素发酵液中的有机酸 ,流动相的流速为 0 6mL/min ,紫外检测波长为 2 10nm。实验结果表明 ,该方法的相对标准偏差为 0 33%～ 0 10 % ,回收率为 99 95 %～ 10 0 0 8%。方法简便、快速、可靠。
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| 关 键 词： | 高效液相法 有机酸 螺旋霉素 发酵液 |
| 文章编号： | 1000-8713(2002)01-0046-03 |
| 修稿时间： | 2001年7月2日 |
Determination of Organic Acids in Fermentation Broth of Spiramycinby High Performance Liquid Chromatography |
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| LI You yuan ,CHEN Chang hua ,TAO Ping.Determination of Organic Acids in Fermentation Broth of Spiramycinby High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2002,20(1):46-48. |
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| Authors: | LI You yuan CHEN Chang hua TAO Ping |
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| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
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| Abstract: | A method for determining organic acids in spiramycin fermentation broth by high performance liquid chromatography is described. The operating conditions were Zorbax 300-SB C18 column (5 microns, 4.6 mm i.d. x 15 cm) at 35 degrees C, 0.01 mol/L phosphoric acid buffer solution (pH 2.32) and methanol as mobile phase at a flow rate of 0.6 mL/min and UV detection at 210 nm. The relative standard deviations were 0.33%-0.10% and the recoveries were 99.95%-100.08%. It's a simple, rapid and accurate method. |
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| Keywords: | high performance liquid chromatography organic acid spiramycin fermentation broth |
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