液相色谱-电喷雾电离质谱与电子轰击质谱联用筛选百合中的甾体皂甙

 利用高效液相色谱 电喷雾电离质谱联用仪 (HPLC/ESI MS)、电子轰击质谱 (EI MS)和半制备型高效液相色谱 ,从卷丹百合中筛选出了两种甾体皂甙 ,其中一种为含有 3个糖基与提果皂甙元的甾体皂甙 ,另一种为含有 3个糖基和薯蓣皂甙元的甾体皂甙。结果表明 :在线的HPLC/ESI MS能够准确快速地提供糖甙类化合物的分子质量和糖链部分的有益信息 ,但对甙元部分提供的信息极少 ;离线的EI MS只需极少量 (1mg～ 2mg)的纯品就能准确地提供甙元部分的有益信息 ,但很难获得糖甙的分子离子峰与糖链部分的信息 ,两者有机地结合起来能快速地从植物中筛选甾体皂甙。

Screening of Steroidal Saponins from the Bulbs of Lilium Brownii var.colchesteri by Combination of High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry and Electron Impact Mass Spectrometry

With the combination of high performance liquid chromatography electrospray ionization mass spectrometry (HPLC/ESI MS) and electron impact mass spectrometry (EI MS), two steroidal saponins, one compound containing three glycosyls and tigogenin and the other one containing three glycosyls and diosgenin, from the bulbs of Lilium brownii var colchesteri in China have been screened In the method, on line HPLC/ESI MS allows us to obtain rapidly useful information about the molecular weight and the glycosyl chain of glycoside without the necessity of isolating individual compounds, but little information about steroidal sapogenins Just with 1 mg to 2 mg of pure sample, off line EI MS allows us to acquire useful information about a steroidal sapogenin of saponins, but it is difficult to obtain the molecular ion peak The combination of HPLC/ESI MS and EI MS is well suitable for rapidly screening steroidal saponins from plants