四苯基卟啉金属螯合物的反相高效液相色谱保留行为研究

 研究了四苯基卟啉金属螯合物(TPP)的高效液相色谱保留行为。在C18柱上试验以各种有机溶剂洗脱金属螯合物，最终选择了甲醇-丙酮的非 水体系作流动相。各螯合物的保留顺序为：ZnTPP＜TPP＜NiTPP＜CuTPP。运用Horvath的疏溶剂理论解释了这种保留行为。文中采用电负性与离子半径之比（EN／ri）作为静电场强的量度，提出 EN／ri值作为四苯基卟啉金属螯合物保留值参数。实验发现，在不同的流动相配比条件下，lnk′与 EN／ri均呈良好的线性关系。

Study on the Retention Behaviour of Metal-Tetraphenyl Porphine Chelates in Reversed-Phase High Performance Liquid Chromatography

The retention behaviour of metal chelates of alpha,beta,gamma,delta-tetraphenylporphine (TPP) was studied in reversed-phase high performance liquid chromatography (RP-HPLC). On C18 column, with various organic solvents as mobile phases, the retention of the chelates tends to increase in the following order: ZnTPP < TPP < NiTPP < CuTPP. This retention behaviour can get a good explanation from Horvath's "solvophobic theory". With the assumption that the electrostatic field strength of metal chelates can be measured by the ratio of electronegativity vs. ionic radius (EN/ri) of central metal ion, EN/ri was established to be an index for the retention behaviour of metal-TPP chelates. It was found that lnk' is in good linear relationship with the EN/ri value.