离子色谱法定量检测酒曲发酵液中的河豚毒素

建立了一种离子色谱定量检测酒曲发酵液中河豚毒素的分析方法。样品经乙腈(含0.1%磷酸)溶液提取和阳离子交换柱净化后,采用离子交换色谱柱分离和紫外检测。在优化的条件下,酒曲样品中的河豚毒素在10～100 mg/L内呈良好的线性关系(r2=0.997),加标回收率为90%～103%,相对标准偏差小于4.9%,检出限(信噪比为3)为1.0 mg/L。结果表明,该方法能达到定量检测的目的。将该方法应用于实际样品的检测,验证了方法的可靠性。河豚毒素初步降解实验发现,随着时间的推移,酒曲中河豚毒素的含量逐渐减少,表明酒曲发酵液对河豚毒素的降解效果显著。

Determination of tetrodotoxin in fermentation broth of distiller's yeast by ion chromatography

A method was developed for the quantitative analysis of tetrodotoxin(TTX) in fermentation broth of distiller's yeast by ion chromatography.After extraction with acetonitrile solution(containing 0.1% phosphoric acid) and purification with an ion-exchange column,the tetrodotoxin was separated by ion chromatography and detected by a ultraviolet-visible(UV-VIS) absorbance detector.The experimental results showed that the tetrodotoxin had a good linearity(r2=0.997) in the range of 10-100 mg/L and the detection l...