固相萃取-高效液相色谱法同时测定调味品中15种工业合成染料

建立了测定调味品中15种工业合成染料的固相萃取-高效液相色谱法(SPE-HPLC)。样品经甲醇-水(1:1, v/v)超声提取、SPE柱净化后用HPLC进行分析,流动相为10 mmol/L乙酸铵溶液(含1%乙酸)和乙腈。实验结果表明15种工业合成染料的分离效果良好,回收率为84.6%～114.2%,相对标准偏差为0.9%～10.3%;检出限为0.05～0.18 mg/kg。该方法操作简单,结果准确,重现性好,可用于同时测定调味品中非法添加的15种工业合成染料。

Simultaneous determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography

A new method was established for the determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography(SPE-HPLC). The samples were extracted by methanol-water (1:1, v/v) and purified by a solid phase extraction column. Then, the chromatographic separation was achieved on a Luna C18 column by linear gradient elution. The mobile phase was 10 mmol/L ammonium acetate-acetonitrile (containing 1% acetic acid). The results showed that the 15 industrial synthetic dyes can be separated efficiently. The recoveries of the 15 industrial synthetic dyes spiked in condiment were between 84.6% and 114.2% with the relative standard deviations of 0.9%~10.3%. The limits of detection of this method was 0.05~0.18 mg/kg for the 15 industrial synthetic dyes. The method is simple, sensitive, accurate, repeatable and can be used for simultaneous determination of the 15 illegally added industrial synthetic dyes.