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高效液相色谱-串联质谱法测定猪肉中的阿维菌素类、地克珠利、妥曲珠利及其代谢物残留
作者姓名:Gong X  Sun J  Dong J  Yu J  Wang H
作者单位:潍坊出入境检验检疫局, 山东 潍坊 261041
基金项目:山东检验检疫局科研基金项目(项目编号:SK201028)
摘    要:建立了猪肉中阿维菌素、伊维菌素、多拉菌素、莫西菌素、乙酰氨基阿维菌素等5种阿维菌素类药物以及地克珠利、妥曲珠利、妥曲珠利砜、妥曲珠利亚砜等4种均三嗪类药物及其代谢物残留的高效液相色谱-串联质谱分析方法。样品采用乙腈提取,ODS粉分散固相萃取净化,经Venusil ASB C18色谱柱(150 mm×2.1 mm, 3.0 μm)分离,电喷雾串联四极杆质谱多反应离子监测方式测定。9种分析物在0.005~0.2 mg/L范围内线性关系良好,相关系数均大于0.990。猪肉基质中9种分析物在0.005、0.01、0.02 mg/kg加标水平下,回收率为73.2%~91.5%,相对标准偏差为12%~17%。该方法稳定、可靠,可满足猪肉中阿维菌素类、均三嗪类药物残留的检测与确证。

关 键 词:高效液相色谱-串联质谱  阿维菌素类药物  地克珠利  妥曲珠利及其代谢物  猪肉
收稿时间:2010-11-18

Determination of avermectin, diclazuril, toltrazuril and metabolite residues in pork by high performance liquid chromatography-tandem mass spectrometry
Gong X,Sun J,Dong J,Yu J,Wang H.Determination of avermectin, diclazuril, toltrazuril and metabolite residues in pork by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography,2011,29(3):217-222.
Authors:Gong Xiaoming  Sun Jun  Dong Jing  Yu Jinling  Wang Hongtao
Institution:Weifang Entry-Exit Inspection and Quarantine Bureau, Weifang 261041, China
Abstract:A method for the determination of avermectin, ivermectin, doramectin, moxidectin, eprinomectin, diclazuril, toltrazuril and its two metabolite residues in pork was developed using QuEChERS method with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample was extracted with acetonitrile and purified through QuEChERS method using ODS as the sorbent. The target compounds were separated on a Venusil ASB C18 column (150 mm x 2.1 mm, 3.0 microm) and detected by HPLC-MS/MS. The linear ranges were 0.005 - 0.2 mg/L and the correlation coefficients were all above 0.990. The average recoveries and the relative standard deviations ranged from 73.2% to 91.5% and from 12% to 17% at the spiked levels of 0.005, 0.01 and 0.02 mg/kg for the 9 analytes in pork matrix. This method is reliable, and suitable for the determination of the residues of avermectin and related compounds in pork.
Keywords:high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)  avermectin  diclazuril  toltrazuril and its metabolites  pork  
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