高效液相色谱-荧光检测法同时测定大鼠不同脑区中的8种单胺类神经递质

采用高效液相色谱-荧光检测法同时测定了大鼠不同脑区中的左旋多巴、去甲肾上腺素、肾上腺素、多巴胺、二羟基苯乙酸、5-羟色胺、5-羟基吲哚乙酸及高香草酸8种单胺类神经递质的含量。样品经组织裂解液(0.60 mol/L高氯酸、0.50 mmol/L Na2EDTA、0.1 g/L L-半胱氨酸的混合水溶液)处理后,冷冻离心得到上清液;上清液中加入高氯酸沉淀剂(1.20 mol/L K2HPO4、2.00 mmol/L Na2EDTA的混合水溶液)处理后,冷冻离心、过滤。在该优化的色谱条件下,8种单胺类神经递质在1.25～5000 μg/L范围内线性关系良好(r＞0.9999),最低检出限在0.20～5.00 μg/L之间,平均回收率在94.83%～99.19%之间,相对标准偏差在0.08%～2.51%之间。该方法具有操作简便、快捷、回收率高、检出限低、分离度好、结果准确可靠等优点,可以用于体内8种单胺类神经递质的同时检测与分析。

Simultaneous detection of 8 monoamine neurotransmitters in the different sections of mouse brains by high performance liquid chromatography with fluorescence detection

A method for the simultaneous detection of L-dihydroxyphenylalanine,norepinephrine,epinephrine,dopamine,3,4-dihydroxyphenylacetic acid,serotonin hydrochloride,5-hydroxyindole-3-acetic acid and homovanillic acid in the different sections of mouse brains was established by using high performance liquid chromatography(HPLC) with fluorescence detection and isocratic elution.Before analysis,the sample was deproteinized by 0.60 mol/L perchloric acid,followed by adjusting pH value of the sample with 1.20 mol/L K2H...