反相高效液相色谱法快速测定复合甜味剂中Aspartam

利用反相高效液相色谱法快速测定各种复合甜味剂中Ａｓｐａｒｔａｍ，用ＯＤＳ短柱（ＵｌｔｒａｓｐｈｅｒｅＸＬ－ＯＤＳ，３μｍ，４．６ｍｍｉ．ｄ．×７．０ｃｍ），以ＣＨ３ＯＨ－０．０２ｍｏｌ／ＬＮＨ４Ａｃ为流动相进行分离，紫外检测器２２０ｎｍ检测，根据色谱保留时间结合对组分不停泵紫外光谱扫描进行定性，用外标法峰高定量，方法最小检出浓度为５μｇ／Ｌ，标准曲线在４０～２００ｍｇ／Ｌ浓度范围内呈线性，相关系数在０．９９９以上，平均添加回收率为９２％，其它复合组分不干扰测定。方法简便、快速、灵敏，非常适合于日常监督检测。

Rapid Determination of Aspartam in Compound Sweetening by Reversed Phase High Performance Liquid Chromatography

A method for rapid determination of Aspartame in compound sweetening by reversed-phase high performance liquid chromatography is presented. Aspartame in compound sweetening was separated in a short column (Ultrasphere XL-ODS, 3 microm, 4.6 mm x 70 mm) by using CH3OH-0.02 mol/L NH4Ac as mobile phase. The flow rate was 0.8 mL/min. Detection was performed with UV detector at 220 nm. The injection volume was 20 microL. It was qualitatively analysed by UV scanning at a wavelength range of 200-350 nm under no-stop flow according to their retention time. Quantitative analysis was carried out by measuring peak height and comparing it with external standard. The minimum detectable amount was 5 microg/L. The linear range of the calibration curve was 40-200 mg/L. The average recovery of Aspartame was 92%. The relative standard deviation was 2.9%. This method is simple, rapid and sensitive.