The retention behavior of arsenic compounds on PRP-X100 column under alkaline condition.

A systematical investigation of the retention behaviour of arsenic compounds (arsenite, arsenate, methylarsonic acid, dimethylarsinic acid, arsenobetaine etc.) on PRP-X100 column under alkaline condition was carried out. The dependence of the retention times of arsenic compounds on pH with two mobile phases: 20 mmol/L ammonium bicarbonate, 2.5 mmol/L p-hydroxybenzoic acid/1.0 mmol/L benzoic acid was studied, and the optimal separations of these five arsenic compounds were achieved.