RP-HPLC测定兔血清和脑脊液中甲硝唑含量

 报告了用RP-HPLC测定免血清和脑脊液中甲硝唑浓度的方法，采用ZorbaxC8柱，NaH2PO4水溶液（0.0075mol／L）-甲醇（4:1，PH4.0）为流动相，氢氯噻嗪为内标，检测波长320um。线性范围0.5～80mg／L，最低检出浓度80μg／L（信噪比为3）。

Determination of metronidazole in serum and cerebrospinal fluid with reversed-phase high performance liquid chromatography]

A reversed-phase high performance liquid chromatographic method for the determination of metronidazole (MTZ) in rabbit serum and cerebro-spinal fluid has been developed. The method is simple and convenient. The analysis was carried out with Zorbax C8 column, NaH2PO4(0.0075mol/L, pH 4.0)-CH3OH(4:11) as mobile phase and UV detector at 320nm, by using hydrochlorothiazide as internal standard. The standard curve is linear over the range from 0.5ng/L to 80.0ng/L in sample with correlation coefficient of 0.9998. The recovery of metronidazole from rabbits serum and cerebrospinal fluid are 97.1% +/- 1.5% and 97.4% +/- 1.6% respectively. The minimum detectable quantity is 80ng/mL in sample (S/N = 3). Under the chromotographic condition, the consistency of MTZ of the serum and cerebrospinal fluids of rabbits were detected separately after taking MTZ (0.1mg/g) orally. The drug-time curve was obtained and a satisfactory result was achieved. The new findings provide conditions for further exploration of the process of pharmacokinetics.