气相色谱法直接测定植物生长素

 建立了一种采用 5 3 0 μm大口径毛细管色谱柱、不经衍生化处理而直接测定吲哚乙酸 (IAA)、吲哚丁酸(IBA)和萘乙酸 (NAA)等植物生长素的气相色谱分析方法。以邻苯二甲酸二丁酯为内标物 ,用 FID检测 ,IAA,IBA和 NAA的相对标准偏差分别为 1 .1 4% ,0 .61 %和 0 .78%。方法简便、快速、准确、重现性好 ,可用于生长素类单组分和混合制剂的质量检测。

Study on the Direct Determination of Auxins by Gas Chromatography

A method for the direct determination of auxins, indole acetic acid (IAA), indole-butyric acid (IBA) and naphthylacetic acid (NAA), by gas chromatography with wide-bore capillary column is described. The gas chromatographic conditions were as follows: HP-1 capillary column, 5 m x 0.53 mm i.d., 2.65 microns film, the column temperature 180 degrees C, the temperature of injector 250 degrees C, the temperature of FID detector 260 degrees C, carrier gas: N2 20 mL/min and dibutyl phthalate was used as internal standard. All components and internal standard were separated in 3 min. The relative standard deviations were from 0.61% to 1.14%. The method is simple, rapid, sensitive, good reproducible and satisfactory.