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甘氨酸衍生化β-环糊精键合硅胶液相色谱固定相的合成与评价
引用本文:冯钰锜,达世禄.甘氨酸衍生化β-环糊精键合硅胶液相色谱固定相的合成与评价[J].色谱,2000,18(3):224-228.
作者姓名:冯钰锜  达世禄
作者单位:武汉大学化学与环境科学学院,湖北,武汉,430072
基金项目:国家自然科学基金资助项目 !( 2 98750 2 0 ),留学回国人员资助费资助项目
摘    要: β-环糊精键合硅胶经对甲苯磺酰化后,与甘氨酸反应得到甘氨酸衍生化β-环糊精键合硅胶固定相(GCDS)。考察了GCDS对位置异构体、丹磺酰化氨基酸异构体、苯丙酸类药物等的分离性能,研究了流动相中甲醇浓度、pH以及柱温对色谱保留行为的影响。

关 键 词:高效液相色谱法  键合硅胶固定相  β-环糊精  甘氨酸

Preparation and Evaluation of Glycine Derivatized β-Cyclodextrin Bonded Silica for High Performance Liquid Chromatography
FENG Yu-qi,DA Shi-lu.Preparation and Evaluation of Glycine Derivatized β-Cyclodextrin Bonded Silica for High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2000,18(3):224-228.
Authors:FENG Yu-qi  DA Shi-lu
Institution:College of Chemistry and Environmental Science, Wuhan University, Wuhan 430072, China. yqfeng@public.wh.hb.cn
Abstract:Glycine derivatized beta-cyclodextrin bonded silica (GCDS) has been prepared for high performance liquid chromatography through the reactions of beta-cyclodextrin bonded silica with tosyl chloride and glycine in sequence. The separation performance of GCDS for positional isomers, dansyl amino acids and phenylpropionic acids was investigated. It was found that nitrophenols could not be eluted with methanol as mobile phase, but could be eluted with methanol-phosphate buffer solution within appropriate time, which was much different from the behavior observed on native beta-cyclodextrin bonded silica. The effect of column temperature on the enantio-separation of dansyl D,L-Phe with GCDS was examined. The results show that the enantio-separation ability of the GCDS decreases with the increase of the column temperature. Other factors such as methanol concentration and pH of the mobile phase influencing the retention behavior of solutes on the GCDS are also discussed.
Keywords:NULL
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