反相高效液相色谱法同时快速测定食品中常见的8种食品添加剂

 采用反相高效液相色谱法测定食品中常见的 8种食品添加剂 :糖精、甜味素、苯甲酸、山梨酸、香兰素、咖啡因、胭脂红和日落黄。实验采用Shim packCLC ODS分析柱 ,以甲醇 乙酸铵 (pH 7 0 ) (体积比为 44∶5 6 )为流动相 ,在UV 2 2 0nm处检测。样品经Carrez试剂处理去除杂质后直接进样 ,一次进样分析仅需 8min。平均回收率为 91 9%～ 10 8 5 % ,相对标准偏差小于 4% (n =5 )。

Simultaneous Rapid Determination of Eight Food Additives in Foods by Reversed-Phase High Performance Liquid Chromatography

A simple and rapid method for the determination of eight food additives by RP-HPLC is described. They were saccharin, aspartame, benzoic acid, sorbic acid, vanillin, caffeine, carmine and sunset yellow. The experiments were carried on Shim-pack CLC-ODS (150 mm x 6.0 mm i.d.) with methanol-20 mmol/L NH4Ac (44:56, V/V; pH 7.0) as the eluent at a flow rate of 1.0 mL/min. The UV detection wavelength was fixed at 220 nm. The food samples, after precipitation of the impurities with Carrez reagent, were injected directly into the HPLC system. The average recoveries of all the eight additives were between 91.9%-108.5%, and the RSDs were lower than 4% (n = 5). The analysis of a single sample required only 8 min. This method has been successfully applied to the routine analysis of these additives in foods.