高效液相色谱法测定大鼠肝过氧化物酶体膜磷脂

 用 HPL C测定了过氧化物酶体膜磷脂的含量。用 Folch法提取膜脂质 ,以 μ-Porasil Si60为固定相 ,以乙腈-甲醇 -磷酸为流动相 ,采用梯度洗脱 ,紫外检测波长 2 0 5 nm。标准回收率 :心磷脂 (C)为 (1 0 3 .97± 1 2 .5 7) % ,磷脂酰肌醇 (PI)为 (88.2 3± 5 .42 ) % ,磷脂酰丝氨酸 (PS)为 (90 .3 3± 6.84) % ,磷脂酰乙醇胺 (PE)为 (84.41±1 0 .2 2 ) % ,磷脂酰胆碱 (PC)为 (89.78± 8.70 ) % ,鞘磷脂 (SM)为 (84.0 4± 1 2 .0 6) %。最小检出限分别为 :C1 2ng,PI 8ng,PS 2 3 ng,PE 4ng,PC 2 3 ng,SM 7ng。线性关系与回收率较好 ,结果令人满意。

Determination of Phospholipid in the Peroxisomal Membrane of Rat Liver by High Performance Liquid Chromatography

A method was employed for the determination of phospholipid in membrane of peroxisomes by high performance liquid chromatography. The separation was performed on a mu-Porasil Si60 column(250 mm x 4.6 mm i.d.) by gradient elution. The phospholipids were detected at 205 nm. The limits of detection for cardiolipin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were 12, 8, 23, 4, 23 and 7 ng respectively and the recoveries were between 84% and 103%. The linear relationship of calibration curves was good (r > 0.91). The phospholipids in peroxisomal membrane of 10 SD rat liver were determined and the results are satisfactory.