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阴离子交换-积分脉冲安培检测法分离测定氨基酸注射液中的氨基酸和葡萄糖
引用本文:于泓,丁永胜,牟世芬.阴离子交换-积分脉冲安培检测法分离测定氨基酸注射液中的氨基酸和葡萄糖[J].色谱,2002,20(5):398-402.
作者姓名:于泓  丁永胜  牟世芬
作者单位:1. 中国科学院生态环境研究中心,北京,100085;哈尔滨师范大学化学系,黑龙江,哈尔滨,150080
2. 中国科学院生态环境研究中心,北京,100085
3. 中国科学院生态环境研究中心,北京,100085;Dionex中国有限公司应用研究中心,北京,100085
摘    要: 用阴离子交换 积分脉冲安培检测法测定了氨基酸注射液中 1 7种氨基酸和葡萄糖。研究了氨基酸和葡萄糖在阴离子交换中的保留行为。采用了优化的水、NaOH和NaAc三元梯度淋洗条件。在优化的梯度淋洗条件和积分脉冲安培检测条件下 ,氨基酸和葡萄糖的检出限为 0 3pmol~ 1 0 3pmol,线性范围约为 2个数量级。样品加标回收率为 88 3 %~ 1 0 4 6 %。方法简单、灵敏、准确。

关 键 词:阴离子交换  积分脉冲安培检测  氨基酸  葡萄糖  氨基酸注射液
文章编号:1000-8713(2002)05-0398-05
修稿时间:2002年3月15日

Determination of Amino Acids and Glucose in Amino Acid Injection by Anion Exchange Chromatography with Integrated Pulsed Amperometric Detection
YU Hong ,DING Yong sheng ,MOU Shi fen.Determination of Amino Acids and Glucose in Amino Acid Injection by Anion Exchange Chromatography with Integrated Pulsed Amperometric Detection[J].Chinese Journal of Chromatography,2002,20(5):398-402.
Authors:YU Hong    DING Yong sheng  MOU Shi fen
Institution:Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, Beijing 100085, China.
Abstract:A direct, sensitive, simple and practical method for the determination of amino acids and glucose in amino acid injection by anion exchange chromatography with integrated pulsed amperometric detection was developed. There is no need for sample pretreatment. The retention behavior of amino acids and glucose on the anion exchange column was investigated using sodium hydroxide and sodium acetate as eluent. When the concentration of sodium hydroxide was changed, the change in the retention times was generally greater for amino acids than for glucose. The difference can be used to improve the separation between amino acids and glucose. When the concentration of sodium acetate was changed, the change in the retention times was generally greater for amino acids containing two carboxyl groups than for amino acids containing only one carboxyl group. The difference can be used to improve the separation between di-carboxylic amino acids and mono-carboxylic amino acids. In order to separate amino acids and glucose, a ternary gradient elution method with water, sodium hydroxide and sodium acetate as mobile phases was employed. The optimized gradient elution condition for the analysis of 17 amino acids and glucose in amino acid injection was obtained. The time of the gradient elution program was 74 min. Under the optimized conditions and the column temperature of 30 degrees C, the detection limits for 17 amino acids and glucose were 0.3 pmol- 10.3 pmol. The calibration graphs of peak area for all the analytes were linear in the ranges about two orders of magnitude. The relative standard deviations (RSDs) (n = 5) were 0.7% - 3.8%. The recoveries of added standard were 88.3% - 104.6%.
Keywords:anion exchange chromatography  integrated pulsed amperometric detection  amino acid  glucose  amino acid injection
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