全扫描-选择离子数据采集技术应用于烟草中生物碱的快速测定

建立了应用气相色谱-质谱(GC-MS)全扫描-选择离子监测(Scan-SIM)数据采集方式同时测定烟草中8种含量相差较大的生物碱的方法,采用Scan模式分析烟碱、降烟碱、新烟碱、去氢新烟碱,同时采用SIM模式分析麦斯明、二烯烟碱、2,3′-联吡啶、可铁宁。结果表明,烟草中各生物碱的回收率为94.8%～98.8%,5次测定的相对标准偏差均小于6%。该方法具有简单、快速、准确的特点,应用于烟草样品测定,结果令人满意。

Rapid determination of alkaloids in tobacco using gas chromatography-mass spectrometry with full scan-selected ion monitoring mode

Simultaneous full scan-selected ion monitoring mode (Scan-SIM) is a new improvement method of data acquisition of gas chromatography-mass spectrometry (GC-MS). A method of determing alkaloids in tobacco was established by GC-MS with Scan-SIM. Nicotine, nornicotine, anabasine and anatabine were determined with scan mode, and myosmine, nicotyrine, 2,3'-bipyridyl and cotinine were determined with SIM mode. The average recoveries of alkaloids ranged from 94.8% to 98.8%, and the relative standard deviations were less than 6.0% (n = 5). Therefore, it is a simple, rapid, accurate method. Tobacco samples picked in different years were determined using this method, and the results were satisfactory.