| 白蛋白在以染料为配基的醋酸纤维滤棒上的等温吸附行为(英文) |
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| 引用本文： | 李京华,王俊德,刘学良.白蛋白在以染料为配基的醋酸纤维滤棒上的等温吸附行为(英文)[J].色谱,2002,20(5):419-422. |
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| 作者姓名： | 李京华 王俊德 刘学良 |
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| 作者单位： | 中国科学院大连化学物理研究所,辽宁,大连,116012 |
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| 基金项目： | ThisworkwassupportedbytheNationalNatureScienceFoundationofChina( 2 0 0 750 31) |
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| 摘 要： | 以醋酸纤维滤棒为基质 ,染料CibacronBlueF3GA为配基 ,合成了一种新的染料亲和介质 ;分别以牛血清白蛋白 (BSA)和人血清白蛋白 (HSA)为对象 ,用静态法进行了吸附实验 ,得到了相应的亲和等温吸附曲线 ;对曲线按Langmuir模型和Freundlich模型分别进行拟合 ;结果表明 ,醋酸纤维滤棒染料亲和介质对BSA和HSA的等温吸附遵循Freundlich模型。采用该亲和介质装柱并分离实际样品人血浆 ,可得到纯化的人血清白蛋白。
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| 关 键 词： | 醋酸纤维滤棒 CibacronBlueF3GA 白蛋白 亲和 Freundlich模型 |
Study of Isothermal Adsorption of Albumin on Acetate Fiber Filter Rod with Dye Affinity Ligand |
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| LI Jing hua,WANG Jun de,LIU Xue liang.Study of Isothermal Adsorption of Albumin on Acetate Fiber Filter Rod with Dye Affinity Ligand[J].Chinese Journal of Chromatography,2002,20(5):419-422. |
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| Authors: | LI Jing hua WANG Jun de LIU Xue liang |
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| Institution: | Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China. |
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| Abstract: | Cibacron Blue F3GA was immobilized onto an acetate fiber filter rod to produce potential affinity matrix. The adsorption isotherms of the prepared matrix for either bovine serum albumin or human serum albumin were investigated. It was experimentally shown that the affinity isothermal adsorption obeyed Freundlich adsorption model. By means of affinity chromatography of human plasma using the prepared matrix, purified human serum albumin was obtained. |
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| Keywords: | acetate fiber filter rod Cibacron Blue F3GA albumin affinity Freundlich model |
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