| 反相离子对高效液相法分离不同种类的磷脂酰胆碱 |
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| 引用本文： | 王智华,卢学清,洪筱坤.反相离子对高效液相法分离不同种类的磷脂酰胆碱[J].色谱,2002,20(3):249-252. |
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| 作者姓名： | 王智华 卢学清 洪筱坤 |
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| 作者单位： | 上海中医药大学,上海,200032 |
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| 摘 要： | 采用反相离子对高效液相 (RP IP HPLC)法分离分析了不同种类的磷脂酰胆碱 (PC) ,柱为PERKIN ELMER/HS 5C18柱 ,流动相为甲醇 乙腈 水 (70∶2 2∶8,体积比 ) (内含 15mmol/L四甲基磷酸铵离子对试剂 ,pH 7) ,流速 2mL/min ,在 2 0 8nm波长处检测 ,该法成功地分离了 7种PC。
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| 关 键 词： | 反相离子对高效液相 磷脂酰胆碱 分子种类 |
| 文章编号： | 1000-8713(2002)03-0249-04 |
| 修稿时间： | 2001年7月2日 |
Separation of Different Phosphatidyl Cholines by Reversed-PhaseIon-Pair High Performance Liquid Chromatography |
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| WANG Zhi hua,LU Xue qing,HONG Xiao kun.Separation of Different Phosphatidyl Cholines by Reversed-PhaseIon-Pair High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2002,20(3):249-252. |
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| Authors: | WANG Zhi hua LU Xue qing HONG Xiao kun |
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| Institution: | Shanghai University of Traditional Chinese Medicine and Pharmacology, Shanghai 200032, China. |
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| Abstract: | A reversed-phase ion-pair high performance liquid chromatographic (RP-IP-HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN-ELMER/HS-5 C18 column (150 mm x 4.6 mm i.d., 5 microns), isocratic elution with mobile phase of methanol-acetonitrile-water (70:22:8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7.0, flow rate of 2.0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method. |
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| Keywords: | reversed phase ion pair high performance liquid chromatography phosphatidyl choline molecular species |
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