蛋白质甲基化修饰的蛋白质组学分析方法新进展

蛋白质的甲基化修饰是一类重要的翻译后修饰。但与磷酸化、糖基化和泛素化等翻译后修饰相比，甲基化修饰的蛋白质组学分析方法开发还是一个较新的研究领域。近几年，由于甲基化修饰在表观遗传调控中的重要作用，这一修饰类型得到了越来越多的关注，相关的分析技术和分析方法也取得了较多进展。其中，基于质谱的蛋白质组学分析方法在甲基化修饰中发挥着关键的作用，实现了这一甲基化修饰的高通量分析。该综述将从甲基化修饰的分离富集、假阳性率控制以及定量蛋白质组学等方面对一些蛋白质甲基化修饰的分析技术和方法的最新进展进行介绍。

Recent advances in proteomic analysis of protein methylation

Protein methylation is one of the most important post-modificational modifications. Compared with the widely studied phoshporylation, glycosylation and ubiquitylation, the methylome analysis is still at nascent stage. In recent years, more and more attentions have been drawn to the researches of protein methylation for its important role in the epigenetic regulation and considerable advances have been achieved. Of them, the MS based proteomic approaches play a key role by achieving the high-throughput analysis of protein methylation. In this review, advances in proteomic analysis of protein methylation are summarized in three aspects:selective enrichment, identification confidence controlling and quantitative analysis.