纳米材料辅助负离子激光解吸电离-飞行时间质谱分析小分子研究进展

基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF MS）作为一种软电离质谱技术，目前已被广泛用于蛋白质、多肽、核酸、聚合物等大分子分析。由于传统有机化合物基质在低相对分子质量（小于700 Da）区域的干扰，该技术在小分子物质分析方面受到很大限制。为克服传统有机化合物基质在低相对分子质量区域的干扰，近年来以纳米材料为代表的无机基质材料备受关注。相对传统有机化合物基质或纳米材料正离子模式，基于纳米材料的负离子激光解吸电离（LDI）有效避免了正离子模式下一种化合物会产生多种加合物的问题，具有图谱简单易于解析、灵敏度高、重现性好等优点。该文综述了近5年来纳米材料负离子LDI-TOF MS技术在小分子分析方面的研究进展，以期拓展该技术在小分子分析方面的应用。

Recent advances of nanomaterials assisted negative ion laser desorption/ionization-time-of-flight mass spectrometry in the analysis of small molecules

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is a soft ionization technique that has been widely used in the analysis of macromolecules, such as proteins, peptides, nucleic acids and polymers. However, it has difficulty in the analysis of small molecules with relative molecular masses below 700 Da because of the interferences from the matrix. To overcome these drawbacks, interference-free nanomaterials assisted laser desorption/ionization (LDI) has been paid more and more attention. Compared with the multiple peaks of one compound obtained in the positive ion mode, nanomaterials assisted negative ion LDI provides only deprotonated ion peak. In this article, recent advances of nanomaterials assisted negative ion LDI in the analysis of small molecules are reviewed.