基于石墨化炭黑预涂覆技术的色谱柱制备和性能评价

采用石墨化炭黑(GCB)预涂覆技术对白酒分析专用色谱柱(LZP-930)的制备方法进行了优化,详细考察了所制备的色谱柱(LZP-930G)的性能参数,并对LZP-930G色谱柱的保留行为进行了评价,探讨了GCB对LZP-930G色谱柱性能的影响。研究发现,LZP-930G色谱柱的柱效、惰性、稳定性和极性均得到了明显提高,从而改善了酸类和醇类组分的分离选择性和色谱峰峰形,实现了白酒中53种易挥发和半挥发性微量组分的同时分离,且各组分的保留时间和峰面积的相对标准偏差分别小于0.63%和4.51%(n=5)。表明GCB预涂覆技术能够用于高性能白酒分析色谱柱的制备方法。

Preparation and performance evaluation of chromatographic column precoated with graphitized carbon black

A preparation method of special chromatographic column (LZP-930) for liquor analysis was optimized by precoating the graphitized carbon black (GCB) on the inner wall of fused capillary column. Characteristics of the prepared chromatographic column (LZP-930G) were investigated in detail. The retention behavior was evaluated, and the effect of the precoated GCB on the performance of the LZP-930G column was discussed. Results revealed that the plate number, stability and polarity were increased obviously by using the LZP-930G column, so the separation selectivity and chromatographic peak shape of acids and alcohols were improved. All the 53 compounds in the mixed liquor standard sample could be separated simultaneously. The relative standard deviations of retention times and peak areas were less than 0.63% and 4.51% (n=5), respectively. The result showed that the GCB precoated technology can be used for the preparation of high performance chromatographic column for liquor analysis.