| 实时直接分析-串联质谱法测定葡萄酒中赭曲霉毒素A |
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| 引用本文： | 宫小明,马荣桧,王洪涛,郭礼强,李凯,吴振兴,赵晗,孙军.实时直接分析-串联质谱法测定葡萄酒中赭曲霉毒素A[J].色谱,2017,35(2):185-190. |
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| 作者姓名： | 宫小明 马荣桧 王洪涛 郭礼强 李凯 吴振兴 赵晗 孙军 |
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| 作者单位： | 1. 潍坊出入境检验检疫局, 山东 潍坊 261041;
2. 山东出入境检验检疫局检验检疫技术中心, 山东 青岛 266002 |
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| 基金项目： | 国家质检总局科研基金资助项目（2013IK177）. |
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| 摘 要： | 建立了葡萄酒中赭曲霉毒素A的实时直接分析-串联质谱(DART-MS/MS)方法。前处理采用乙酸乙酯提取,二甲基十八碳硅烷粉(ODS)粉分散固相萃取净化,采用DART-MS/MS检测,同位素稀释内标法定量。结果表明:在1.0、2.0、10μg/kg 3个加标水平下,回收率为88.7%~105.7%,RSD为8.5%~12.8%,定量限为0.5μg/kg。该方法具有简单、快速、灵敏度高等特点,能满足葡萄酒中赭曲霉毒素A检测的要求。
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| 关 键 词： | QuEChERS 同位素稀释内标 实时直接进样分析 串联质谱法 赭曲霉毒素A 葡萄酒 |
| 收稿时间： | 2016-07-07 |
Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry |
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| GONG Xiaoming,MA Ronghui,WANG Hongtao,GUO Liqiang,LI Kai,WU Zhenxing,ZHAO Han,SUN Jun.Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry[J].Chinese Journal of Chromatography,2017,35(2):185-190. |
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| Authors: | GONG Xiaoming MA Ronghui WANG Hongtao GUO Liqiang LI Kai WU Zhenxing ZHAO Han SUN Jun |
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| Institution: | 1. Weifang Entry-Exit Inspection and Quarantine Bureau, Weifang 261041, China;
2. Technical Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China |
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| Abstract: | A method for the determination of ochratoxin A (OTA) in wine was developed using direct analysis in real time-electrospray ionization tandem mass spectrometry (DART-MS/MS). The sample was extracted with ethyl acetate and purified by votexing with ODS powder, detected using DART-MS/MS. The results showed that the average recoveries ranged from 88.7% to 105.7% with the RSDs of 8.5%-12.8% at three spiked levels of 1.0, 2.0, and 10 μ g/kg. The LOQ was 0.5 μg/kg. The proposed method was accurate, rapid and reliable, which can be successfully applied to the qualitative and quantitative analysis of ochratoxin A in wine. |
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| Keywords: | QuEChERS isotope dilution internal standard direct analysis in real time (DART) tandem mass spectrometry (MS/MS) ochratoxin A (OTA) wine |
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