常用十字花科药用植物中芥子碱的分布研究

十字花科植物中的芥子碱是一种非常有价值的天然抗氧剂,在抗衰老药物的研究中受到了许多学者的重视。为了考察芥子碱在我国药用植物中的分布情况，采用反相高效液相色谱法测定了我国常用的4种十字花科药用植物白芥子、莱菔子、葶苈子、黄芥子中该成分的含量。采用的色谱柱为Alltima Phenyl柱(250 mm×4.6 mm i.d.,5 μm);流动相:3%醋酸溶液和乙腈的混合溶液,梯度洗脱;检测波长:326 nm;柱温:25 ℃。该法快速，准确度高，重现性好，方法的建立不仅为上述4种药材的质量监测提供了有效的定量分析方法，而且对于芥子碱的深入研究具有重要意义。

Study of Distribution of Sinapine in Commonly Used Crude Drugs from Cruciferous Plants

Sinapine, an important natural antioxidant, is often obtained from cruciferous plants. Many scientists are interested in it because of its great potential in the field of antiageing drugs. Thus, the distribution of sinapine in 4 commonly used crude drugs from cruciferous plants (Semen Sinapis Albae, Semen Brassicae Junceae, Semen Raphani and Semen Lepidii) was investigated. The determination was performed by reversed-phase high performance liquid chromatography (RP-HPLC) using an Alltima Phenyl column (250 mm x 4.6 mm i. d., 5 microm). A gradient elution with mobile phase consisting of (A) 3% acetic acid in water and (B) acetonitrile was used. The detection wavelength was set at 326 nm and the column temperature was kept at 25 degrees C. It is an accurate, rapid and reproducible method. The results are informative for the research of a quality control tool for these natural drugs.