| 高效液相色谱法测定贝类中的软骨藻酸 |
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| 引用本文： | 卫锋,程晻,宫静宏,唐守亭.高效液相色谱法测定贝类中的软骨藻酸[J].色谱,2001,19(3):248-250. |
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| 作者姓名： | 卫锋 程晻 宫静宏 唐守亭 |
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| 作者单位： | 大连理工大学, |
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| 摘 要： | 介绍了用反相高效液相色谱 (RP HPLC)测定贝类中软骨藻酸的方法。样品以V(甲醇 )∶V(水 ) =1∶1的溶液提取 ,经LC SAX强阴离子柱固相萃取净化 ,用RP HPLC定量分析。方法的最小检出限为 0 2 μg/ g ,在 1 0mg/L～ 2 5 0mg/L范围内有良好的线性关系 ,测定结果准确 ,重现性好 ,回收率大于 96 %。
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| 关 键 词： | 高效液相色谱法 固相萃取 软骨藻酸 贝类 |
| 文章编号： | 1000-8713(2001)03-0248-03 |
| 修稿时间： | 2000年10月18 |
High Performance Liquid Chromatographic Determination of Domoic Acid in Shellfish
Samples |
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| WEI Feng ,CHENG Fang ,GONG Jing hong ,TANG Shou ting.High Performance Liquid Chromatographic Determination of Domoic Acid in Shellfish
Samples[J].Chinese Journal of Chromatography,2001,19(3):248-250. |
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| Authors: | WEI Feng CHENG Fang GONG Jing hong TANG Shou ting |
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| Institution: | Liaoning Entry-Exit Inspection and Quarantine Bureau of China, Dalian 116001, China. |
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| Abstract: | Domoic acid in shellfish samples was determined by HPLC with DAD/UV detector at 242 nm. Samples were extracted with methanol-water followed by clean-up of the extracts with strong anion exchange solid phase extraction cartridge(3 mL LC-SAX). Zorbax SB-C18 column, 150 mm x 4.6 mm i.d., and mobile phase of acetonitrile-0.1% aqueous trifluoroacetic acid(13:87, V/V) were used for the assay. The quantitative analysis was performed with external standard. The calibration curve of domoic acid was linear in the range of 1.0 m/L-25.0 mg/L and the detection limit was ca. 0.2 microgram/g. |
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| Keywords: | high performance liquid chromatography solid phase extraction domoic acid shellfish |
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