嘌呤衍生物在葫芦［6］脲单轮烷键合固定相上的色谱行为

在反相和正相色谱模式下,研究了几种嘌呤衍生物在葫芦［6］脲单轮烷键合硅胶固定相上的高效液相色谱行为,并在反相模式下与ODS固定相进行了比较,考察了流动相中甲醇含量、流动相pH值和离子强度对嘌呤化合物保留的影响。研究结果表明:在反相模式下,嘌呤化合物与葫芦［6］脲单轮烷键合相之间存在多种相互作用,除疏水作用外,分离过程中还存在与ODS不同的色谱分离机制。在正相条件下,多作用力的色谱分离机制同样存在。葫芦［6］脲单轮烷键合相与溶质之间存在疏水、氢键、π-π和偶极-偶极等多种作用力,协同作用提高了固定相对嘌呤化合物的分离选择性。

Chromatographic Behavior of Purine Derivatives on Cucurbit [6] uril Monorotaxane-Bonded Silica Stationary Phase by High Performance Liquid Chromatography

The chromatographic behavior of purine derivatives on a new cucurbit 6] uril monorotaxane-bonded silica stationary phase (CB6MRBS) was studied using high performance liquid chromatography both under normal and reversed-phase modes. A comparative study of this phase with ODS was carried out as well. The influences of methanol content, pH value and ionic strength of mobile phases were investigated in detail. The results showed that various interactions occurred between the analytes and CB6MRBS besides hydrophobic interaction under reversed-phase were different from those on ODS. According to the chromatographic analysis, the separation mechanism of multiple interactions also exists under normal phase. The CB6MRBS can provide various sites for analytes, such as the hydrophobic, hydrogen-bonding, pi-pi and dipole-dipole interactions. Separations of purine derivatives on CB6MRBS were achieved with satisfaction due to the various retention mechanisms both under reversed- and normal phase modes.