胶束电动毛细管色谱检测鱼肉中的七种生物胺

建立了一种利用胶束电动毛细管色谱同时检测鱼肉中组胺、腐胺、2-苯乙基胺、尸胺、色胺、亚精胺及精胺7种生物胺的方法。样品经6%过氯酸萃取后,由苯甲酰氯衍生化,以含0.06 mol/L脱氧胆酸钠的0.02 mol/L硼酸(pH 9.2)-甲醇(体积比为95∶5)混合液为电泳介质,电泳电压25 kV,温度25 ℃,检测波长214 nm,在12 min内实现了7种生物胺的完全分离。7种生物胺的浓度与其峰面积在一定的范围呈良好的线性关系,检出限除组胺为15 μg/g外,其余均为5 μg/g。迁移时间和峰面积的日内、日间相对标准偏差均小于5%。该法用于海鱼中7种胺类物质含量的测定,结果令人满意。

Determination of Seven Biogenic Amines in Fish Using Micellar Electrokinetic Capillary Chromatography

A method for the determination of histamine, 2-phenylethylamine, tryptamine, putrescine, cadaverine, spermidine and spermine in fish using micellar electrokinetic capillary chromatography (MECC) was developed. Seven biogenic amines (BAs) were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol (95 : 5, v/v) was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges. The detection limit of histamine was 15 microg/g and those of the other amines were 5 microg/g. The developed method was successfully employed for the simultaneous determination of 7 BAs in fish sample with satisfactory results.