超高效液相色谱法同时测定蜂胶中的12种活性成分

采用超高效液相色谱法同时测定了蜂胶中类黄酮、阿魏酸、咖啡酸苯乙酯等12种活性成分。蜂胶样品用甲醇稀释,超声波提取,样液过滤后以Acquity BEH C18柱(100 mm×2.1 mm,1.7 μm)为分离柱,0.4%磷酸水溶液和甲醇为流动相,梯度洗脱,采用二极管阵列检测器检测。整个分离过程在9.5 min内完成。以加标蜂胶样品做添加回收测定,12种化合物的回收率为90.1%～104.3%,相对标准偏差为2.12%～4.90%。该方法为评价蜂胶质量提供了一种新的检测方法,已应用于实际蜂胶样品的测定。

Determination of Twelve Active Compounds in Propolis Using Ultra-Performance Liquid Chromatography

In this study, a fast, selective and sensitive ultra-performance liquid chromatographic method (UPLC) with photodiode array detection (DAD) was developed to determine 12 active compounds, such as flavonoids, ferulic acid, caffeic acid phenethyl ester, etc. in propolis. The propolis sample was diluted with methanol and extracted by sonication. The gradient separation was achieved within 9.5 min by using an Acquity BEH C18 column (100 mm x 2.1 mm, 1.7 microm) with 0.4% phosphoric acid aqueous solution and methanol as the mobile phase. The experimental parameters were optimized and adapted to analyse propolis samples from different production areas in China. The spiked recoveries were 90.1% - 104.3% with relative standard deviations of 2.12% -4.90%. This method can be used as a novel method for quality control of propolis. The results are validated through testing 106 propolis samples from various production areas.