茶叶及茶多酚中儿茶素的高效液相色谱分析方法研究

 筛选出HypersilBDSC18和ZorbaxSBC18两种适合同时分离茶叶和茶多酚中 7种儿茶素和咖啡因的反相柱。采用甲醇 水 醋酸 (或三氟醋酸 )作流动相 ,分别以等强度洗脱和梯度洗脱 (均在 30min内 )分离测定了我国 6种不同产地茶叶样品和 3种茶多酚样品中 7种儿茶素的含量。考察了 7种儿茶素和咖啡因的保留值与流动相组成及柱温的关系 ,优化了色谱条件及样品前处理方法。用电喷雾电离质谱 (ESI MS)定性确认没食子儿茶素没食子酸酯(GCG)和儿茶素没食子酸酯 (CG)两组分 ,并用高效液相色谱制备两对照品用于定量分析。

Study on the Analytical Methods of Catechins in Tea and Green Tea Polyphenol Samples by High Performance Liquid Chromatography

Hypersil BDS C 18 and Zorbax SB C 18 , suitable to separate simultaneously seven kinds of catechins and caffeine, were screened out from seven brands of reversed phase columns Mobile phase was a solution of methanol water acetic acid (or trifluoro acetic acid) Seven kinds of catechins in tea samples from six places in China and three green tea polyphenol(GTP) samples from different producers were separated and determined in 30 min by isocratic and gradient elutions The effects of mobile phase components and temperature of column on retention parameters of catechins and caffeine are reviewed Chromatographic conditions and pretreatment methods of samples were optimized Gallocatechin gallate(GCG) and (-) catechin gallate(CG) were identified by electrospray ionization mass spectrometry(ESI MS) and prepared by high performance liquid chromatography for quantitative analysis The other catechins, (-) epigallocatechin(EGC),( ) catechin(D C),(-) epicatechin(EC),(-) epigallocatechin gallate(EGCG),(-) epicatechin gallate(ECG) were identified with standards.