松花粉干预卵巢早衰大鼠肝脏的广泛靶向代谢组学分析

卵巢早衰是妇科领域的常见病，中医认为卵巢早衰与肝肾的正常与否息息相关，通过药食两用物质对肝脏代谢的调理是治疗卵巢早衰的一种重要手段。该研究基于超高效液相色谱-三重四极杆质谱(UHPLC-MS/MS)建立的广泛靶向代谢组学技术，探讨破壁松花粉对环磷酰胺诱导的卵巢早衰模型大鼠肝脏代谢的影响，旨在通过测定对照组、模型组、雌激素阳性对照组及施以不同剂量的松花粉干预组的SD大鼠肝脏组织中代谢物的含量变化，结合主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)等多元统计方法揭示松花粉干预卵巢早衰大鼠肝脏代谢的作用机制。通过正、负离子模式共检测出687种肝脏代谢物，PCA与OPLS-DA显示环磷酰胺诱导的模型组能够与对照组、阳性对照组、松花粉干预组等组别之间的代谢物较好的分离。通过单变量分析中的t检验(p<0.05)、变异倍数(FC>2或<0.5)与多变量分析中变量投影重要性(VIP值)>1相结合对差异代谢物进行筛选。与对照组相比，模型组SD大鼠肝脏中的32个生物标志物含量显著升高，28个生物标志物含量显著降低，主要涉及α-亚麻酸代谢、维生素B6代谢、嘌呤代谢、赖...

Pseudotargeted metabolomics analysis of pine pollen intervention in the liver of premature ovarian failure rats

Premature ovarian failure (POF) is a prevalent gynecological disease. In traditional Chinese medicine, it is believed that POF is directly related to abnormal function of the liver and kidneys. As such, regulation of the liver metabolism through the use of medicinal and edible substances is important for the treatment of POF. Pine pollen, a traditional Chinese medicinal and edible pollen variety, contains various active substances, such as sex hormones and phytohormones, which have been used to inhibit inflammation, regulate the immune system, and protect reproductive tissues. Using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS), this study examined the influence of pine pollen on the liver metabolome of cyclophosphamide-induced POF model Sprague Dawley (SD) rats. The variations in the metabolites present in the liver tissue of control SD rats, model SD rats, and SD rats treated with various doses of pine pollen or estrogen were analyzed using principal component analysis (PCA) in combination with orthogonal partial least squares discriminant analysis (OPLS-DA) and other multivariate statistical methods to reveal the mechanism of pine pollen intervention in the livers of POF SD rats.