反相高效液相法测定血清中的佐匹克隆

 建立了测定血清中佐匹克隆的反相高效液相法 (外标法 )。血样用正丁基氯提取后进行分离。采用的柱为LiChroCART 12 5 4柱 (LiChrospher 6 0RPselectB填料 ,5 μm ,12 5mm× 4mmi d ) ,流动相为乙腈 0 0 2mol/LKH2 PO4缓冲溶液 (体积比为 2 0∶80 ) ,紫外检测波长为 2 5 4nm。当佐匹克隆在血清中的添加质量浓度分别为 4 0 0 μg/L ,16 0 0 μg/L和6 4 0 0 μg/L时 ,血清中佐匹克隆的回收率分别是 (73 4± 3 2 ) % ,(82 2± 4 1) %和(90 3± 4 5 ) %。方法的最低检出限为 15 μg/L。方法适用于法庭毒物分析 ,简便、快速。

Determination of Zopiclone in Serum by Reversed-PhaseHigh Performance Liquid Chromatography

A reversed-phase high performance liquid chromatographic method (external standard method) was developed for the determination of zopiclone in serum. After the selective extraction with n-butyl chloride, this compound was chromatographed on a LiChroCART 125-4 column packed with LiChrospher 60 RP select B(5 microns) using acetonitrile-monopotassium phosphate (20:80, V/V) as mobile phase. The eluting compound was measured by an ultraviolet detector at 254 nm. Spiked with zopiclone of 400 micrograms/L, 1,600 micrograms/L and 6,400 micrograms/L in serum, the recoveries of zopiclone were (73.4 +/- 3.2)%, (82.2 +/- 4.1)% and (90.3 +/- 4.5)% respectively. The detection limit of the method was 15 micrograms/L. The method is simple and rapid for the determination of zopiclone in forensic toxicology.