| 高效液相色谱-串联质谱法同时测定鳗鱼和虾中残留的33种喹诺酮和磺胺类药物 |
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| 引用本文： | 王志杰,冷凯良,孙伟红,宁劲松,翟毓秀.高效液相色谱-串联质谱法同时测定鳗鱼和虾中残留的33种喹诺酮和磺胺类药物[J].色谱,2009,27(2):138-143. |
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| 作者姓名： | 王志杰 冷凯良 孙伟红 宁劲松 翟毓秀 |
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| 作者单位： | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| 基金项目： | 农业部科研重大专项项目，国家高技术研究发展计划(863计划)，基本科研业务费专项项目 |
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| 摘 要： | 建立了鳗鱼和虾中33种喹诺酮类(QNs)和磺胺类(SAs)药物残留量的高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。以氘代试剂为内标,样品经酸性乙腈萃取后,用正己烷脱脂,旋转蒸发浓缩,采用LC-MS/MS选择反应监测(SRM)正离子模式测定,同时对鳗鱼和虾中的33种QNs和SAs进行定性和定量。33种QNs和SAs的检出限(S/N=3)为1.0 μg/kg,定量限(S/N=10)为2.0 μg/kg;在10.0～200.0 μg/L时目标物的峰强度与质量浓度的线性关系良好(r>0.99);平均回收率为66%～123%。该法简便快捷,降低了分析成本,也在一定程度上实现了药物残留的快速检测。
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| 关 键 词： | 高效液相色谱-串联质谱 氘代试剂 内标法 喹诺酮类药物 磺胺类药物 多残留 鳗鱼 虾 |
| 收稿时间： | 2008-7-17 |
| 修稿时间： | 2008-9-24 |
Simultaneous determination of 33 quinolone and sulfonamide residues in eels and shrimps by high performance liquid chromatography-tandem mass spectrometry |
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| WANG Zhijie,LENG Kailiang,SUN Weihong,NING Jinsong,ZHAI Yuxiu.Simultaneous determination of 33 quinolone and sulfonamide residues in eels and shrimps by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography,2009,27(2):138-143. |
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| Authors: | WANG Zhijie LENG Kailiang SUN Weihong NING Jinsong ZHAI Yuxiu |
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| Institution: | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: | The method for the simultaneous determination of 33 quinolone(QN) and sulfonamide(SA) residues in eels and shrimps was developed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The deuterium substituted reagents which were used as internal standards were added to the sample before the extraction.The sample was extracted with acidified acetonitrile,cleaned-up by hexane,and concentrated with a rotary evaporator.The mass spectrometer was operated in the positive ion mode using se... |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) deuterium substituted reagents internal standard method quinolones sulfonamides multi-residues eels shrimps |
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