高效液相色谱法分离测定人参中的6种人参皂甙

 采用梯度法，以乙腈-水溶液为流动相，用高效液相色谱法分离人参中的6种主要人参皂甙，并采用紫外检测器检测，在203 nm处测定4种人参样品。该方法在25 mg/L～300 mg/L的范围内有良好的线性关系，回收率高于80%。

Determination of Six Ginsenosides in Panax Species by High Performance Liquid Chromatography

A method for the determination of six major ginsenosides in panax species by high performance liquid chromatography (HPLC) with UV detection at 203 nm is described. The six major ginsenosides, which were reported to account for more than 90% of the total ginsenosides content of the panax species' root, were successfully separated using gradient HPLC. The column to be used was a Cosmosil-C18 reversed-phase, 4.6 mm i.d. x 250 mm, 5 microns (Japan), connected with a C18 guard column and the mobile phase was acetonitrile and water. The calibration curves of the six ginsenosides were all linear over the range of 25 mg/L-300 mg/L with good correlation coefficients (> 0.99). The recoveries for the ginsenosides were all above 80%. For real samples and recovery study, the major ginsenosides were ultrasonically extracted by methanol. The extracts were evaporated to 1 mL, under vacuum, at about 50 degrees C and then diluted by methanol. Just prior to HPLC analysis, the sample solutions were filtered using a C18 cartridge. This method has been successfully applied to the analysis of four commercial panax samples.