亚种间杂交稻内源激素的高效液相测定法

 建立了一种快速、提取率高的从植物中提取内源激素的样品处理方法 ,并研究了高效液相法测定亚种间杂交稻的 4种内源激素 :赤霉素 (GA3 )、3 吲哚乙酸 (IAA)、玉米素 (Z)和脱落酸 (ABA)的条件。采用WatersC18反相柱 (4 6mmi.d .× 2 5 0mm ,5 μm) ,SPD 6AV紫外检测器。以甲醇 水 乙酸 (体积比为 45∶5 4 2∶0 8)溶液为流动相 ,流速 1 0mL/min ;进样量 2 0 μL ;检测波长 2 5 4nm ;选用外标法进行定量测定。其回收率高 ,检出限分别为GA3 0 5mg/L ,IAA 0 1mg/L ,Z 0 3mg/L ,ABA 0 0 3mg/L。该法快速、灵敏、准确。

High Performance Liquid Chromatographic Determination of Internal Hormones in Inter-Subspecific Hybrid Rice

A simple, rapid and perfect extraction and determination method for the internal hormones (gibberellin acid (GA3), indole-3-acetic acid (IAA), zeatin (Z), abscisic acid (ABA)) in inter-subspecific hybrid rice with reversed-phase high performance liquid chromatography has been developed. The chromatographic conditions were as follows: a Waters C18 column (4.6 mm i.d. x 250 mm, 5 microns), an SPD-6AV detector set at 254 nm and the mobile phase was a mixture of methanol, water and acetic acid (45:54.2:0.8, volume ratio). The lower detection limits were 0.5 mg/L for GA3, 0.1 mg/L for IAA, 0.3 mg/L for Z and 0.03 mg/L for ABA.