固相微萃取-高效液相联用分析环境水样中的痕量■

 应用固相微萃取与高效液相联用技术 (SPME HPLC)分析了环境水样中的痕量 艹屈 。对SPME的条件如萃取时间、萃取温度、离子强度、解吸方式、解吸溶剂、解吸时间和HPLC条件进行了优化 ,建立了SPME HPLC分析环境水样中痕量 艹屈 的方法 ,并将其用于分析自来水、雨水、矿泉水和江水等实际水样。方法的线性范围为 0 0 13μg/L～ 3 0 μg/L ,检出限为 2 7ng/L ,相对标准偏差 (RSD ,n =6 )为 5 6 % ,回收率为 10 3 2 %～ 119 3%。该方法适合于环境水样中痕量 艹屈 的分析 ,体现了SPME在样品前处理中快速、灵敏、简单、无溶剂的特点。

Determination of Trace Chrysene in Environmental Waterby Solid-Phase Microextraction Coupledwith High Performance Liquid Chromatography

A rapid method for the determination of trace chrysene in environmental water by solid-phase microextraction (SPME) coupled with high performmnce liquid chromatography (HPLC) was developed. The experimental conditions of SPME, such as extraction time, extraction temperature, effect of ion strength, desorption time, desorption solution, desorption mode and the analytical conditions of HPLC were optimized. The optimal conditions were room temperature, 1,100 r/min of stirring rate, 30 min of extraction time, 3 min of desorption time, and methanol as the desorption solution. Methanol was used as the mobile phase on a C18 reversed phase chromatographic column. The flow rate was 1 mL/min. The detection wavelength was 266 nm on a UV-Vis detector. The linear range was from 0.013 microgram/L to 3.0 micrograms/L, the detection limit was 2.7 ng/L, and the relative standard deviation (RSD) was 5.6%. The method was used for the determination of trace chrysene in tap water, mineral water, rain water and river water. The recoveries were from 103.2% to 119.3%, the RSDs were from 4.8% to 10.2%. The method is fast, convenient, sensitive, solvent-free, and suitable for the determination of trace chrysene in environmental water.