高效液相色谱法测定斯帕沙星注射液

 采用高效液相色谱法对斯帕沙星注射液中的主成分进行定量分析。色谱条件 :色谱柱为美国Waters公司的SymmetryC18(5 μm ,15 0mm× 3 9mmi d )不锈钢柱 ,检测波长为 2 98 8nm ,柱温 30℃ ,流动相为 0 2 %KH2 PO4缓冲液 (pH 3 2 ) CH3 CN CH3 OH(体积比为 80∶15∶5 )的混合溶液 ,进样量为 10 μL ,流速为 1 0mL/min。在该色谱条件下的测定结果是 :以斯帕沙星峰计算柱效为 15 30 0理论板数 /m ;斯帕沙星的线性范围为 39 94mg/L～ 199 6 8mg/L ,相关系数r =0 9999;平均加样回收率为 10 0 1% ,RSD为 0 72 %

Quantitative Analysis of Sparfloxacin Injection by High Performance Liquid Chromatography

Analytical method of the quantitative determination of sparfloxacin injection by HPLC is described. The analytical conditions were as follows. A Waters Symmetry C18(5 microns, 150 mm x 3.9 mm i.d.) column was used as the analytical column. The detection wavelength was UV-298.8 nm. The column temperature was 30 degrees C. The mobile phase was 0.2% KH2PO4 buffer (pH 3.2)-CH3CN-CH3OH (80:15:5, volume ratio) and the flow-rate was 1.0 mL/min. The injection volume was 10 microL. The linear range (the peak area vs. the mass concentration of sparfloxacin) was from 39.94 mg/L to 199.68 mg/L, and the correlation coefficient was 0.9999. The average recovery of sparfloxacin was 100.1% (n = 5), and its RSD was 0.72%. The RSDs of continuous injections, within day injections per 2 hours and between day injections in three days were 0.19%, 0.14% and 0.13% respectively. The above analytical results show that this method has good precision and stability. It is a rapid, sensitive and accurate method for the analysis of sparfloxacin.