高效液相色谱法同时测定化妆品中的7种磺胺及甲硝唑和氯霉素

建立了化妆品中7种磺胺(磺胺醋酰、磺胺吡啶、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺甲氧嘧啶、磺胺间甲氧嘧啶、磺胺甲基异唑)和甲硝唑及氯霉素的高效液相色谱测定方法。样品经0.1%甲酸水溶液-乙腈(体积比为8∶2)混合液超声提取后进行液相色谱分析。方法的定量检测限为3～80 μg/g,7种磺胺在20～200 μg/mL时,甲硝唑及氯霉素在40～400 μg/mL时方法的线性关系良好(r≥0.9993)。加标回收率为83.8%～105.3%(7种磺胺的添加水平为50 μg/mL和150 μg/mL，甲硝唑及氯霉素的添加水平为100 μg/mL和300 μg/mL),其相对标准偏差均小于5%。

Simultaneous Determination of Seven Sulfonamides and Metronidazole and Chloramphenicol in Cosmetics by High Performance Liquid Chromatography

A method for the simultaneous determination of seven sulfonamides (sulfacetamide, sulfapyridine, sulfamerazine, sulfamethazine, sulfamater, sulfamonomethoxine, sulfamethoxazole) and metronidazole, chloramphenicol in cosmetics has been developed by high performance liquid chromatography with a photodiode array detector (HPLC-PDA). The chromatographic column Atlantis dC18 (150 mm x 4.6 mm, 5 microm) was used with acetonitrile-0.1% formic acid (2:8, v/v) aqueous solution as mobile phase at a flow rate of 1.0 mL/min. Samples were extracted by mobile phase and detected by HPLC-PDA at 268 nm. The limits of quantification were 3 - 80 microg/g. The correlation coefficients of linear calibration curves were over 0. 999 3 within the sulfonamide concentration range of 20 - 200 microg/mL and metronidazole and chloramphenicol concentration range of 40 - 400 microg/mL. Average recoveries were 83.8% - 105.3% at the spiked levels of 50 microg/mL and 150 microg/mL for sulfonamides, and 100 microg/mL and 300 microg/mL for metronidazole and chloramphenicol. The relative standard deviations were below 5%. This method can be routinely used for the determination of seven sulfonamides and metronidazole, chloramphenicol in cosmetics.