高效液相色谱法测定杜仲叶中的绿原酸

 采用高效液相色谱法测定杜仲叶中绿原酸的含量。所用色谱柱为Shim-packCLC-ODS柱,以巯嘌呤为内标,以甲醇-0.01mol/L磷酸二氢钾缓冲液(体积比为25∶75;pH3.9)为流动相,UV检测波长为328nm。在上述色谱条件下,当绿原酸质量浓度为25～300mg/L时,以绿原酸对照品溶液与内标液的峰面积比为纵坐标(Y),绿原酸的质量浓度为横坐标(X),二者呈良好的线性关系,线性方程为Y=8.0368X+1.275×10-3,r=0.9999;平均回收率为99.89%,RSD=1.45%(n=6)。

Quantitative Determination of Chlorogenic Acid in Eucommia Leaf by High Performance Liquid Chromatography

An HPLC method for the quantitative determination of chlorogenic acid in Eucommia leaf was investigated. The sample was extracted with methanol solution containing 5% fomic acid and then analyzed by HPLC. The operating conditions were Shim-pack CLC-ODS column (6.0 mm i.d. x 150 mm, 5 microns) at 40 degrees C, mobile phase of methanol-0.01 mol/L KH2PO4(25:75, V/V; pH 3.9) at 1 mL/min and UV detection at 328 nm. Mercaptopurine was selected as the internal standard. There was a good linear relationship between the response (area ratio of chlorogenic acid to mercaptopurine) and the concentration of sample in the range of 25-300 mg/L for chlorogenic acid. The corresponding regression equation was Y = 8.0368X + 0.001275, r = 0.9999. The average recovery was 99.89% and RSD was 1.45%(n = 6). The results showed that this method is simple, specific and accurate.