基于拟靶向液相色谱-质谱联用的胃癌患者血清代谢组分析

胃癌是一种高发的恶性肿瘤，是癌症相关死亡的第二大病因。早期筛查是提高患者生存率的有效手段，但目前临床上尚缺乏实现胃癌无创筛检的可靠标志物。本研究采用了基于液相色谱-质谱联用的拟靶向代谢组学方法分析了20例胃癌患者及40例正常人血清代谢组，以期发现新的潜在代谢标志物。代谢组数据的主成分分析和偏最小二乘法数据分析结果显示，胃癌患者与健康人群的血清代谢组存在明显的差异，结合非参数检验进一步筛选并定性出57个差异代谢物。其中二氢胆固醇经验证组样本验证，具有成为胃癌代谢标志物的潜力。本研究在发现胃癌的潜在代谢标志物的同时，也为胃癌患者代谢分型提供了重要的科学依据。

A serum metabolomics study of gastric cancer based on pseudotargeted liquid chromatography-mass spectrometry approach

Gastric cancer is one of the most common malignant and the second most frequent cause of cancer-related mortality in the world. Noninvasive screen methods including tumor markers are intensively needed in the clinic. To discover metabolic markers for gastric cancers, the sera metabolic profiles of 20 gastric cancer (GC) patients and 40 healthy controls were analyzed by liquid chromatography coupled with mass spectrometry (LC-MS) using a pseudotargeted approach. The results of principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) indicated that obvious classification could be observed between GC and the healthy controls. According to the results of non-parametric statistical test, 57 ions were identified among all the differential metabolic features. Of all the identified differential metabolites, dihydrocholesterol provide good diagnostic performance of GC, which was also validated by another cohort including the 20 gastric cancer patients and the 39 healthy controls. With this robust pseudotargeted approach, our study provides both potential tumor markers and the basis for the metabolic phenotype of gastric cancers.